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王建军,冯莉赟,申虎飞 刘 佼.超声波辅助的植物转化方法的研究进展[J].农学学报,2017,7(11):6-13..Advance in Plant Transformation Method Assisted by Ultrasonic[J].Journal of Agriculture,2017,7(11):6-13
超声波辅助的植物转化方法的研究进展
Advance in Plant Transformation Method Assisted by Ultrasonic
投稿时间:2016-12-29  修订日期:2017-03-17
DOI:10.11923/j.issn.2095-4050.cjas16120034
中文关键词: 超声波  辅助  植物  转化方法
英文关键词: Ultrasonic  Assisstance  Plant  Transformation method
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基金项目:国家转基因重大专项“抗病虫、抗除草剂转基因玉米新品种选育”(2016ZX08003001-002-003);山西省科技攻关项目“多抗耐密型玉米新种 质创制及新品种选育”(20140311002-1)。
作者单位E-mail
王建军 山西农业科学院隰县农业试验站 隰县 041399 wangjianjun1123@126.com 
冯莉赟 山西农业科学院隰县农业试验站 隰县 041399 fengliyun1246@126.com 
申虎飞 刘 佼 山西农业科学院隰县农业试验站 隰县 041399 853205702@qq.com 
中文摘要:
      为了探索超声波处理在植物转化中的功效及潜在发展能力,从超声波辅助植物转化的原理入手,归纳了方法的验证、超声波处理的受体材料和超声波处理对外源DNA的影响;总结了前人在超声波辅助植物转化方面的研究进展,进而分析了方法自身的优缺点及存在问题。指出超声波辅助植物转化方法虽然处于初级发展阶段,但超声波辅助的植物转化仍然是一个操作简单、省时省力、适用范围广的植物转化方法,文中还提出了存在的问题且给出了相应的解决对策;通过分析认为超声波辅助的植物转化方法具有广阔的发展前景。
英文摘要:
      To evaluate the efficiency and potential development capacity of ultrasonic treatment for plant transformation, we started with the principle of ultrasonic- assisted plant transformation, summarized the validation of method, the receptor materials of ultrasonic treatment and the effects of ultrasonic treatment on exogenous DNA. Then, the research progresses in ultrasonic-assisted plant transformation were reviewed, and the advantages as well as disadvantages and existing problems of the method were discussed. Our results indicated that although the development of ultrasonic-assisted plant transformation was still in the primary stage, it demonstrated that the method was a simple, time-saving, labour-saving and widely applied plant transformation approach. Moreover, we proposed respective solution to the existing problems. Taken together, we consider that ultrasonic-assisted plant transformation method has broad development prospect.
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